Figure 4.

Comparison of the Information Provided by Population and Single Cell Lipid Profiling Approaches

(A) PLS-DA scores plot of population level lipid profiling comparing wild-type and A53T mutants (Mu).

(B) Boxplot of the abundance of PC 36:2 in wild-type and A53T mutants (Mu); p value calculated using generalized linear model.

(C) PLS-DA scores plot of single cell lipid profiling comparing wild-type and A53T mutants (Mu).

(D) Boxplots of the abundance of PC 36:2 in each biological replicate, p value calculated using generalized linear model.

(E) Beanplot showing the relative distribution of the abundance of PC 36:2 in each biological replicate cells where each line represents the abundance measured in a cell and the width of the outline represents the density of samples.

(F) Beanplot showing the relative distribution of the abundance of PC 36:2 in combining all wild-type and all mutants where each line represents the abundance measured in a cell and the width of the outline represents the density of samples. All p values were calculated using generalized linear models.

Mu, SNCA-A53T mutant dopaminergic neurons; PC, glycerophosphocholine; PLS-DA, partial least squares-discriminant analysis.