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scTHI workflow. Description of scTHI functionalities. (A) In the first step a single sample enrichment analysis based on the Mann-Witney-Wilcoxon gene set test (mww-GST) [39] with a collection of 295 gene immune and stromal signatures is used to nominate the identity of cells of the microenvironment. (B) In the second step, given 2 clusters of cells, the most significant L–R interactions are identified by assigning a score to all 2,548 collected pairs of ligands and receptors.
