Table 2.

Primers and probes of the candidate genes for DNA methylation analyses.

Gene	Primers	Probes
SLC1A6	F: 5′-GGAAACAGAGAAGCCTGG-3′ R: 5′-CTCAGGAAGCGCTCATTA-3′	M: 5′-TTCGCAGCCTTCGCCATC-3′ U: 5′-TTCACAGCCTTCGCCATC-3′
BHLHB9	F: 5′- TTAGTGTGGTTTTTTTTAATTTT−3′ R: 5′- TAACATAACAACCACCAC−3′	M: 5′- AACAAACGAACAACTAAAAACCC-3′ U: 5′- AACAAACAAACAACTAAAAACCC−3′
LYNX1	F: 5′- TTAGTTTAGTTAGGTTGGAAA-3′ R: 5′- TTCACATTATCTACACTTCTC-3′	M: 5′- ACCTCGACCTAAACTCAAACCTCAC-3′ U: 5′- ACCTCAACCTAAACTCAAACCTCAC−3′
CAV2	F: 5′- TGAGTGGTTAGTAGGTTAA-3′ R:5′- ACAATCACATCTATAATTATCTTAC-3′	M: 5′- CACGAAAACAAAACCCTAACACAAAACC-3′ U: 5′- CACAAAAACAAAACCCTAACACAAAACC-3′
PCSK9	F: 5′- TTGTGTTTATTATAGAAT-3′ R: 5′- TTTATACTACAAAAATTC-3′	M: 5′- AACGTCATATAAATACATTCAA-3′ U: 5′- AACATCATATAAATACATTCAA-3′

F, forward; R, reverse; M, Methylated; U, Un-Methylated.