Table 4.
Comparison of methylation copy numbers of the candidate genes in three groups (Unadjusted: ± SD; Adjusted: ± SE).
| HMAQ group (n = 168) | LMAQ group (n = 39) | Health control group (n = 105) | F | p | ||||
|---|---|---|---|---|---|---|---|---|
| Unadjusted | Adjusted | Unadjusted | Adjusted | Unadjusted | Adjusted | |||
| CAV2a, b, c | 3.43E5 ± 5.08E5 | 3.45E5 ± 4.10E4 | 1.05E6 ± 9.83E5 | 1.05E6 ± 8.51E4 | 1.78E5 ± 2.65E5 | 1.77E5 ± 5.18E4 | 38.673 | 0.000 |
| BHLHB9b, c | 1.09E5 ± 6.42E4 | 1.09E5 ± 4.34E3 | 1.18E5 ± 7.39E4 | 1.18E5 ± 9.01E3 | 4.56E4 ± 2.69E4 | 4.56E4 ± 5.49E3 | 47.075 | 0.000 |
| PCSK9b, c | 2.39E6 ± 1.62E6 | 2.40E6 ± 1.41E5 | 1.85E6 ± 1.40E6 | 1.84E6 ± 2.93E5 | 5.89E6 ± 2.23E6 | 5.89E6 ± 1.78E5 | 136.373 | 0.000 |
| SLC1A6b, c | 3.11E4 ± 1.86E4 | 3.10E4 ± 2.01E3 | 3.03E4 ± 2.06E4 | 3.05E4 ± 4.18E3 | 7.05E4 ± 3.61E4 | 7.06E4 ± 2.55E3 | 80.477 | 0.000 |
| LYNX1b, c | 3.01E5 ± 1.59E5 | 3.01E5 ± 1.15E4 | 3.27E5 ± 2.33E5 | 3.26E5 ± 2.38E4 | 1.18E5 ± 6.85E4 | 1.18E5 ± 1.45E4 | 56.107 | 0.000 |
HMAQ group (high MA addictive quality group): MA abusers conformed to the diagnostic criteria of MA dependence according to the SCID-I/P with drug abuse frequency≥10 times and drug abuse time≥1 year; LMAQ group (low MA addictive quality group): MA abusers did not conform to the diagnostic criteria of MA dependence according to the SCID-I/P with drug abuse frequency≥10 times and drug abuse time≥1 year.
No linear regression between the interaction of age * group and the methylation level of SLC1A6 (F = 0.637, p = 0.530), CAV2 (F = 1.971, p = 0.141), LYNX1 (F = 0.004, p = 0.996), PCSK9 (F = 1.184, p = 0.308), BHLHB9 (F = 0.534, p = 0.587), respectively were found. Covariance analysis with age as covariance was used to compare the methylation copy numbers of the candidate genes in three groups, and Bonferroni post-hoc test was used to do the pairwise comparisons.
The pairwise comparison between HMAQ group and LMAQ group, p < 0.05.
The pairwise comparison between HMAQ group and health control group, p < 0.05.
The pairwise comparison between LMAQ group and health control group, p < 0.05.