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. 2020 Oct 23;7:603817. doi: 10.3389/fmolb.2020.603817

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Copyright © 2020 Miao, He, Qi and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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M2 macrophages and regulatory T cell population analysis. (A,B) Flow cytometric analysis of cells in the mice periodontal tissue in each group. The M2 macrophages were gated with F4/80⁺ and CD206⁺; the Treg cells were gated with CD4⁺ and Foxp3⁺. Both the ratios of F4/80⁺ and CD206⁺ cells and CD4⁺ and Foxp3⁺ cells are lower in the periodontitis group and higher in the healthy group and cell injection group. (C) Western blot analysis of IL-6 and ARG-1 protein in the gingival tissue. The statistical significance was determined by P-value (***P < 0.001, ****P < 0.0001).