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. 2020 Oct 23;11:561722. doi: 10.3389/fphys.2020.561722

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Copyright © 2020 Agócs, Pap, Sugár, Tóth, Turiák, Veréb, Kemény, Tulassay, Vannay and Szabó.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of optimal sodium (Na⁺) concentrations with in vitro cell viability assays: (A) LDH cytotoxicity assay, and (B) MTT cell viability assay performed on cultured human dermal fibroblast cells after 24 h. Na⁺ concentration in the control media was 150 mM. n = 6 wells/group. One-way ANOVA test was performed. Data are presented as mean ± SD. Authors state that the number of independent experiments in reference to cell viability (MTT assay) was 3, and that the one shown is 1 representative of the corresponding repeats.