Table 2.
Primer sequences and annealing temperatures (Ta) used during MS-HRM analysis, amplicon length, and number of CpG sites for each gene.
| Gene | Primer sequences | Ta | Amplicon length (bp) | CpG sites |
|---|---|---|---|---|
| MGMT | F 5′-GCGTTTCGGATATGTTGGGATAAGT-3′ R 5′-AACGACCCAAACACTCACCAAA-3′ | 58° | 110 | 12 |
| MLH1 | F 5′-AGTTTTAAAAACTGAATTAATAGGAAGAG-3′ R 5′-ACTACCCGCTACCTAAAAAAATATAC-3′ | 56° | 81 | 5 |
| CDKN2A | F 5′-CGGAGGAAGAAAGAGGAGGGGT-3′ R 5′-CGCTACCTACTCTCCCCCTCT-3′ | 62° | 93 | 7 |
| RASSF1A | F 5′-TCGGGTTTTATAGTTTTTGTATTTAGGTTTT-3′ R 5′-CCTCCCCCAAAATCCAAACTAA-3′ | 60° | 87 | 7 |