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. 2020 Oct 23;11:577795. doi: 10.3389/fgene.2020.577795

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Copyright © 2020 Sun, Yao, Wu, Deng, Ma, Jin, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondrial oxidative stress is activated in M7 cybrids. (A) Mitochondrial ROS was determined in M8 and M7 cybrids by MitoSOX. Relative mitochondrial ROS levels were normalized to M8-1 cybrid. (B) Representative Western blot of mitochondrial quality control proteins, including GRP75, HSP60, LONP1, and CLPP, from whole-cell extracts of M8 and M7 cybrids. ACTIN was used as a loading control. Relative protein levels were normalized to M8-1 cybrid. Data are presented as the mean ± SD from at least 3 independent tests per experiment. *P < 0.05; **P < 0.01.