Figure 4.

Analysis of Sig1R protein levels after exposure to SA4503, PRE084 and (+)-PTZ. These 661W cells were treated with SA4503 (3 and 25 µM), PRE084 (3 and 25 µM), or (+)-PTZ (3 and 25 µM) for 24 hours. Immunoblotting was performed to determine the level of Sig1R protein after treatment with Sig1R ligands compared with no treatment. β-Actin served as protein loading control. Data are mean ± SEM of two independent experiments with three repetitions per assay. Two-way ANOVA, significant differences (*P < 0.05; ns, not significant) are in reference to control (nontreated) cells.