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. 2020 Nov 3;61(13):6. doi: 10.1167/iovs.61.13.6

Figure 7.

Figure 7.

Expression of Prickle family in the developing human tear duct. (A–L) All panels are in situ hybridization (purple) followed by p63 immunostaining (red) at gestation week 8 (GW8). Brackets and arrows indicate areas of the developing tear duct (TD) and in situ hybridization signal. A, C, and E are three consecutive sections with 30 µm intervals. B, D, and F are same sections respective to A, C, and E. A to F PRICKLE1/p63. G and H) PRICKLE2/p63. I and J) PRICKLE3/p63. K and L PRICKLE4/p63. M and N) The 3D reconstruction of the human embryonic tear duct with p63 staining. The tear duct is drawn as green, whereas the head surface epithelium is drawn as red. M Top view of the developing tear duct. N Inside view of the developing tear duct. cj, conjunctiva; NLD, nasolacrimal duct; SLC, superior lacrimal canaliculus; ILC, inferior lacrimal canaliculus. (O) Schematic illustration of developing TD in the context of the GW8 embryonic head. Red dashed lines and black arrows indicate sectioning region and direction, respectively. man, mandibulary.