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A–C
Single optical slices from three embryos at E5.75. All embryos are in lateral orientation, with the anterior side to the left as defined by the position of AVE cells (Hex‐GFP, in green). Membranes are marked by tdTomato (in grey). Scale bars represent 50 μm. (A′, B′, C′) Upper row: number of rosettes normalized by the total number of cells in the epiblast region in focus expressed in percentage; mid row: frequency of rosettes, normalized by the area of the epiblast region in focus and the time of observation; bottom row: frequency of apical cell rounding, normalized by the area of the epiblast region in focus and the time of observation. Data show consistently a higher number of rosettes in the posterior side compared with the anterior and lateral side, both in absolute and normalized values. Time interval between acquisitions is 7 min, and interval between Z‐sections is 1 μm.
Data information: values are shown as Mean ± SEM, and number of time points analysed per embryo are
n (A′) = 12,
n (B′) = 19 and
n (C′) = 10. Normality was assessed using a Shapiro–Wilk test followed accordingly by either an ANOVA test and paired two‐tailed
t‐tests or Kruskal–Wallis test and Mann–Whitney tests. *
P‐value ≤ 0.05, **
P‐value ≤ 0.01 and ***
P‐value ≤ 0.001.
Source data are available online for this figure.
