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. 2020 Oct 5;21(11):e50944. doi: 10.15252/embr.202050944

Figure 5. Non‐apical divisions occur in the posterior epiblast before and at the onset of gastrulation.

Figure 5

  • A
    3D reconstruction from whole‐mount lightsheet imaging of an E5.75 Hex‐GFP (green, anterior marker) embryo, stained for nuclei (DAPI, grey) on the left, mitosis (Phh3, magenta) and F‐actin (Phalloidin, grey) on the right. Scale bar: 50 μm.
  • B
    Z‐slice (left) and 3D reconstruction (right) from whole‐mount lightsheet imaging of an E6.25 embryo stained for a posterior marker (Eomesodermin, cyan), mitosis (Phh3, magenta) and F‐actin (Phalloidin, grey). Scale bar: 50 μm.
  • A′, B′
    Mitotic Index (Phh3/DAPI) in percentage at the anterior and posterior regions of E5.75 (A′) and (B′) E6.25 embryos. Posterior region includes the PS region, as it could not be precisely discriminated. Normality was assessed using a Shapiro–Wilk test followed by two‐tailed t‐test or Mann–Whitney test. ns, non‐significant.
  • A″, B″
    Ratio of non‐apical mitosis (non‐apical Phh3 over total Phh3, normalized by number of nuclei) in percentage in the anterior and posterior region of E5.75 (A″) and E6.25 (B″) embryos. Normality was assessed using a Shapiro–Wilk test followed by Mann–Whitney tests. **P‐value ≤ 0.01 and ***P‐value ≤ 0.001.
Data information (A′, B′, A″, B″): values are shown as Mean ± SEM. E5.75: n embryos = 11; n cells: anterior = 1,491, posterior = 1,548. E6.75: n embryos = 7; n cells: anterior = 1,602, posterior = 1,629. Source data are available online for this figure.