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Temperature dependence of the 2H NMR spectra of a ternary POPC/PSM/Chol mixture (molar ratio 4/4/2) hydrated to 50 wt% aqueous buffer (K2PO4 20 mM, 100 mM NaCl, 0.1 mM EGTA, pH 7.4) in the absence (black spectra in the left columns) and in the presence of 9 mol% 5-HT (colored spectra in the right columns) at various temperatures as indicated. (A–D) 2H NMR spectra were recorded for each component of the mixture: (A) POPC-d31, (B) PSM-d31, (C) Chol-d6, (D) 5-HT-d4.
