Table 3.
Sequences of primers used for cloning of gRNA plasmids and for genotyping of colonies
Name | Forward primer sequence | Reverse primer sequence |
---|---|---|
Reno gRNA1 | CACCGAAGCTACTCCCTATCCGGCGT | TAAAACGCCGGATAGGGAGTAGCTTC |
Reno gRNA2 | CACCGCAAACCAATGGCATCGCGTGT | TAAAACACGCGATGCCATTGGTTTGC |
Bahcc1 gRNA | CACCGTGTGCGAAGCCATCGGCAGGT | TAAAACCTGCCGATGGCTTCGCACAC |
Reno genotyping | GGTTGGGGGATAACTGTATGAA | CTTCTCGGGGAAATAGACCCT |
Bahcc1 genotyping | GCATTTGCTATCGGAGCG | CATTGCACAGGGAAATGAAAC |