Figure 2. pA‐DamID can map NL interactions and correlates with microscopy observations.

• A
  Example of raw pA‐DamID data tracks from 2 million HAP‐1 cells for Lamin B2, H3K27me3, and the Dam control from a single experiment. Sequenced reads are counted in 20 kb bins and normalized for library size.
• B
  Same pA‐DamID tracks of Lamin B2 and H3K27me3 as in (A) but after normalization to the Dam‐only control (to correct for accessibility and amplification biases) and log₂ transformation.
• C
  Correlation between the median peripheral enrichment of ^m6A‐Tracer determined by confocal microscopy and enrichment of sequencing reads within LADs in HAP‐1 cells. The LAD definition is based on conventional Lamin B1 DamID data. Every point represents a single pA‐DamID experiment for which both microscopy and sequencing data were generated, colored by the antibody used. The blue line represents a linear model with a standard error confidence interval in gray. The red dashed line represents the diagonal. The Pearson correlation coefficient was converted to a t‐statistic and a one‐sided t‐test with n‐2 degrees of freedom was used to determine statistical significance.
• D
  Comparisons of pA‐DamID genome‐wide data (bin size 20 kb) for different lamin antibodies in hTERT‐RPE cells: Lamin B1 vs. Lamin B2 (left panel) and Lamin B2 vs. Lamin A/C (right panel). n denotes the number of independent biological experiments that were averaged for each data track. The blue line represents a linear model. The red dashed line represents the diagonal.