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. 2020 Oct 5;21(11):e50283. doi: 10.15252/embr.202050283

Figure 5. The NDIME/ MEF2C axis promotes the neural gene expression programs and regulates numerous ASD risk genes expression.

Figure 5

  1. Summary of the DEGs in the NDIME‐knockout cells compared with control cells at day 5 of neural differentiation.
  2. Analysis of significant GO terms for genes that were downregulated by at least two‐fold in the NDIME‐knockout cells at day 5 of neural differentiation (hypergeometric test, P < 0.05).
  3. Summary of the DEGs in the MEF2C‐knockdown cells compared with control cells at day 5 of neural differentiation.
  4. Analysis of significant GO terms for genes that were downregulated by at least 2‐fold in the MEF2C‐knockdown cells compared with control cells at day 5 of neural differentiation (hypergeometric test, P < 0.05).
  5. Pathway enrichment analysis for the DEGs in the NDIME‐knockout cells compared with control cells at day 5 of neural differentiation.
  6. Pathway enrichment analysis for the DEGs in the Mef2c‐knockdown cells compared with control cells at day 5 of neural differentiation.
  7. Overlap of DEGs between the NDIME‐knockout cells and the MEF2C‐knockdown cells.
  8. Analysis of significant GO terms for genes that were dysregulated in (G) (hypergeometric test, P < 0.05).
  9. Heatmap illustrating the expression of selected neurectoderm genes and mESCs‐specific genes that were shown as log2 FPKM in Ctrl, NDIME −/−, shCtrl and shMef2c mESCs at day 5 of neural differentiation. Each lane corresponds to an independent biological sample.
  10. qRT–PCR analysis of representative co‐upregulated and co‐downregulated genes in (G) at day 5 of neural differentiation.
  11. Overlapping between co‐regulated genes in (G) and gene sets of interest (hypergeometric test, P = 0.0174).
  12. Relative expression of selectively downregulated ASD risk genes from the DEGs of the NDIME‐knockout cells compared with control cells at day 5 and from the DEGs of the MEF2C‐knockdown cells compared with control cells at day 5.
Data information: Data in (J and L) are represented as mean ± SEM, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired two‐tailed Student's t‐test).