Figure 1. Irgm1 and Irgm2 but not Irgm3 limit the production of inflammatory cytokines in response to LPS in vivo .

1. WT, Irgm1 ^−/−, Irgm2 ^−/−, and Irgm3 ^−/− mice (n = 4 mice/genotype) were injected i.p. with LPS (8 mg/kg). Serum was collected 4 h post‐injection (hpi) and concentration of various cytokines determined via a preconfigured Luminex multiplex panel. Relative concentration (fold change relative mean of WT) is shown for the indicated cytokines (absolute cytokine concentrations of same experiment are shown in Fig EV1).
2. WT (n = 9), Irgm1 ^−/− (n = 7), Irgm2 ^−/− (n = 9), and Casp1 ^−/− Casp11 ^−/− (n = 7) mice were injected i.p. with LPS (8 mg/kg). Serum was collected 4 hpi and concentration of IL‐1β, IL‐18, and TNFα was measured via ELISA.

Data information: Data shown are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 for indicated comparisons or comparison between WT and indicated genotype by one‐way ANOVA with Dunnett's multiple comparison test. N.S, non significant.