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. 2020 Oct 9;21(11):e50214. doi: 10.15252/embr.202050214

Figure 3. atgl‐1 autonomously regulates neuronal LD dynamics.

Figure 3

  1. The expression pattern of atgl‐1 viewed by P atgl‐1 ::GFP transcriptional fusion reporter. P atgl‐1 ::GFP expression is high in intestine. Relatively low fluorescent signals are found in nerve ring, motor neuron commissure, ventral nerve cord, and tail. Scale bar: 20 μm.
  2. atgl‐1 is expressed in neurons. There is no fluorescent signal when P atgl‐1 ::GFP1‐10 and P rab‐3 ::GFP11 are expressed separately. When P atgl‐1 ::GFP1‐10 and P rab‐3 ::GFP11 are co‐expressed, the GFP signals are found in the head region, tail, and ventral nerve cord, where most neuronal cell bodies are located. The white lines mark the outline of worms. Scale bar: 50 μm.
  3. atgl‐1 is expressed widely in neurons. The left panels depict the whole worm. Scale bar: 50 μm. The insets in the right panels are enlarged views of the ventral nerve cord in the white dotted boxes of left panels. The signals of mCherry and GFP merge very well. Scale bar: 20 μm.
  4. Visualizing LDs in head neurons with the xdIs109 reporter in different genetic backgrounds. The accumulation of LDs in head neurons of atgl‐1(xd314) mutants can be rescued by expressing wild‐type atgl‐1 in neurons (P unc‐119 ::atgl‐1), but not in intestine (P vha‐6 ::atgl‐1), glia (P hlh‐17 ::atgl‐1), or hypodermis (P ajm‐1 ::atgl‐1). Scale bar: 5 μm.
  5. Quantification of the number of LDs in head neurons in different genetic backgrounds. Each dot represents one worm. The data were analyzed using one‐way ANOVA with Dunnett's multiple comparison test. Asterisks denote significant differences as compared to the control xdIs109. ns: not statistically significant. ****P < 0.0001. Data show mean ± SEM. n ≥ 7.
  6. Neuron‐specific knockout of atgl‐1 causes LD accumulation in neurons, similar to atgl‐1(xd314) mutants. Scale bar: 5 μm.
  7. Quantification of the number of LDs in head neurons in different genetic backgrounds. Each dot represents one worm. The data were analyzed using one‐way ANOVA with Dunnett's multiple comparison test. Asterisks denote significant differences as compared to the control xdIs109. t‐Test was used between the groups under the crossbars. ns: not statistically significant. ****P < 0.0001. Data show mean ± SEM. n ≥ 9.