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. 2020 Oct 9;21(11):e50214. doi: 10.15252/embr.202050214

Figure EV3. Manipulating ROS scavengers slightly influences the protective effect of lipolysis mutations or PUFA mutations on neurodegeneration caused by mec‐4(d) .

Figure EV3

  • A
    The percentage of worms that have three or more surviving touch neurons in different genetic backgrounds under different conditions. Feeding with the antioxidant NAC slightly increases the survival of neurons in mec‐4(d) worms. Feeding with the antioxidant vitamin C significantly increases the survival of neurons in fat‐4(wa14); mec‐4(d) worms. The data were analyzed using one‐way ANOVA with Bonferroni's multiple comparison test. Asterisks signify significant differences between the groups under the crossbars. ns: not statistically significant. *P < 0.05, ****P < 0.0001. Data show mean ± SEM. Number of experiments n ≥ 4, with at least 50 animals per strain analyzed in each experiment.
  • B
    The percentage of worms that have three or more surviving touch neurons in different genetic backgrounds. sod‐2 mutation does not enhance neurodegeneration in mec‐4(d), atgl‐1(xd314); mec‐4(d), fat‐4(wa14); mec‐4(d) and atgl‐1(xd314); fat‐4(wa14); mec‐4(d). The data were analyzed using one‐way ANOVA with Bonferroni's multiple comparison test. Data show mean ± SEM. ns: not statistically significant. Number of experiments n ≥ 5, with at least 50 animals per strain analyzed in each experiment.
  • C
    The percentage of worms that have three or more surviving touch neurons in different genetic backgrounds. Using the touch neuron‐specific promoter Pmec‐4 to drive sod‐1, sod‐2, sod‐3, or sod‐4 overexpression in touch neurons does not significantly affect neurodegeneration in mec‐4(d). The data were analyzed using one‐way ANOVA with Bonferroni's multiple comparison test. Data show mean ± SEM. ns: not statistically significant. Each dot stands for one experiment, with at least 50 animals per strain analyzed in each experiment.
  • D, E
    The percentage of worms that have three or more surviving touch neurons in different genetic backgrounds. Overexpression of glutathione peroxidases in touch neurons does not generally increase the survival of neurons. The data were analyzed using one‐way ANOVA with Bonferroni's multiple comparison test. Data show mean ± SEM. ns: not statistically significant. Number of experiments n ≥ 5, with at least 50 animals per strain analyzed in each experiment.