Figure EV2. EWS depletion induces PARP1 expression in mouse embryo and cell lines.

• A, B
  Quantitative real‐time PCR was carried out to investigate the expression level of NAD⁺ salvage pathway genes using E17.5 day embryos liver (A) and cell lines (B). Data represented as mean ± SEMs, and obtained from three different cell line and embryo liver. Significance determined by Student's t‐test, two‐tailed, *P < 0.05, ***P < 0.001.
• C
  Immunostaining of 17.5 days embryo tissues (mid‐brain, heart, liver, and skin) with anti‐PARP1‐antibody. Insert shows higher magnification. Scale bar indicates 200 μm (left).