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. 2020 Sep 10;21(11):e50078. doi: 10.15252/embr.202050078

Figure 2. hMENA expression in tumor and stroma of PDAC and NSCLC tissues.

Figure 2

  1. Representative images of immunofluorescence of Pan‐hMENA (yellow) and α‐SMA (red) in the primary PDAC tissue of patient #138 from whom high hMENAΔv6 CAFs were obtained. Nuclei were stained with 4′,6‐diamidino‐2-phenylindole (DAPI). Scale bar: 50 μm. The inset of the dashed area is provided on the right as a zoomed‐in and cropped fluorescence image. Scale bar: 20 μm. αSMA‐positive CAFs are also positive for Pan‐hMENA (arrow).
  2. Representative images of immunofluorescence of Pan‐hMENA (yellow) and α‐SMA (red) in NSCLC case #484 from whom high hMENAΔv6 CAFs were obtained. Nuclei were stained with 4′,6‐diamidino‐2-phenylindole (DAPI). Scale bar: 50 μm. The inset of the dashed area is provided on the right as a zoomed‐in and cropped fluorescence image. Scale bar: 20 μm. As in A, α‐SMA signal is evident in stromal cells which are also positive for Pan‐hMENA (arrow).
  3. Boxplots showing the mean mRNA expression of ENAH gene (hMENA) for 22 patients in the 10 groups of cell types (Peng et al, 2019; total number of cells: 38,487, mean of cells for patient: 1,603). Shown in each boxplot are the median value (horizontal line), 25th–75th percentiles (box outline), and highest and lowest values within 1.5× of the interquartile range (vertical line). Expressions from each cell‐type group were compared to all other groups by using Mann–Whitney U‐test (two‐sided) and P values were adjusted for multiple testing using the Benjamini–Hochberg method. Stromal cell‐type groups with significantly up‐regulated ENAH expression respect to other stromal groups are: Fibroblast, ***q = 0.0002; Stellate, ***q = 1.5e‐07. Non‐stromal cell‐type groups with significantly up‐regulated ENAH expression with respect to other non‐stromal groups are: Ductal 1, **q = 0.0013.
  4. Boxplots showing the mRNA expression of ENAH gene (hMENA) in the seven groups of cell types from (Lambrechts et al, 2018) (total n = 52). Shown in each boxplot are the median value (horizontal line), 25th–75th percentiles (box outline), and highest and lowest values within 1.5× of the interquartile range (vertical line). Cell expression from each group were compared to all other stromal/not-stromal cells by using Mann–Whitney U‐test (two‐sided) and P values were adjusted for multiple testing using the Benjamini–Hochberg method (Fibroblast group vs other stromal groups, **q = 0.0022; Other comparisons, q > 0.05).