Figure 1. Chronic cold exposure induces oxidative metabolism in BAT.

Male mice, housed at 30°C or 6°C for 10 days, were intraperitoneally (IP) administered with [U‐¹³C]glucose (2 g/kg). 15 minutes after injection, BAT was harvested for metabolic enrichment assay.

• A
  m+6 glucose enrichment in serum and BAT.
• B
  Relative glucose abundance in serum and BAT.
• C
  Illustration of the intracellular carbon transition in metabolic tissues after [U‐¹³C]glucose administration.
• D, E
  Metabolic ¹³C enrichments in BAT of male mice are shown as m+3 glycolytic intermediates (D), m+2 TCA cycle intermediates (E).

Data information: n = 10, data are represented as the mean ± SD. Statistical analysis was performed using two‐tailed Student's t‐test, *P < 0.05. 3PG, 3‐Phosphoglycerate; PEP, phosphoenolpyruvate; αKG, α‐ketoglutarate. Source data are available online for this figure.