Figure EV1. Chronic cold exposure induces glucose metabolism in BAT.

Mice, housed at 30°C or 6°C for 10 days, were administered with [U‐¹³C]glucose (2 g/kg, IP). 15 minutes after injection, BAT was harvested for metabolic enrichment assay.

1. To directly compare the change of enrichment between different metabolites, the relative metabolic ¹³C enrichments in BAT of male mice are normalized to the average enrichment of each metabolite in 30°C group.
2. Relative abundance of glycolytic and TCA cycle intermediates in BAT.
3. The enrichment and relative abundance of glyceraldehyde 3‐phosphate (G3P).
4. The enrichment of palmitate.

Data information: n = 10, data are represented as the mean ± SD. Statistical analysis was performed using two‐tailed Student's t‐test, *P < 0.05. Source data are available online for this figure.