Figure 2. Chronic cold exposure induces oxidative metabolism in sWAT.

• A
  Representative immunofluorescence staining shows UCP1 (green), perilipin (red), and DAPI (blue) in sWAT of mice housed at 6°C.
• B–G
  Male mice, housed at 30°C or 6°C for 10 days, were administered with [U‐¹³C]glucose (2 g/kg, IP). 15 min after injection, sWAT and gWAT were harvested for metabolic enrichment assay. Metabolic ¹³C enrichments in sWAT of male mice are shown as m+6 glucose (B), m+3 glycolytic intermediates (C), and m+2 TCA cycle intermediates (D). Metabolic ¹³C enrichments in gWAT of male mice are shown as m+6 glucose (E), m+3 glycolysis intermediates (F), and m+2 TCA cycle intermediates (G).
• H
  Plasma ¹³CO₂ enrichment, after normalizing to the [U‐¹³C]glucose tracer enrichment.

Data information: n = 10 mice per group for (B‐G), n = 7‐8 mice per group for (H), data are represented as the mean ± SD. Statistical analysis was performed using two‐tailed Student's t‐test, *P < 0.05. Source data are available online for this figure.