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. 2020 Oct 12;21(11):e50085. doi: 10.15252/embr.202050085

Figure EV2. Chronic cold exposure does not induce oxidative metabolism in liver or muscle.

Figure EV2

Mice, housed at 30°C or 6°C for 10 days, were administered with [U‐13C]glucose (2 g/kg, IP). 15 minutes after injection, liver and muscle were harvested for metabolic enrichment assay.
  1. m+6 glucose enrichment in liver.
  2. Metabolic 13C enrichments in liver are shown as m+3 glycolysis intermediates, m+2 TCA cycle intermediates. GAP, glyceraldehyde 3‐phosphate; DHAP, dihydroxyacetone phosphate.
  3. After normalizing to the glucose enrichment in the liver of each mouse, the relative metabolic 13C enrichments were shown as m+3 glycolysis intermediates and m+2 TCA cycle intermediates.
  4. m+6 glucose enrichment in muscle.
  5. Metabolic 13C enrichments in muscle are shown as m+3 glycolysis intermediates, m+2 TCA cycle intermediates.
Data information: n = 10, data are represented as the mean ± SD. Statistical analysis was performed using two‐tailed Student's t‐test, *P < 0.05. Source data are available online for this figure.