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. 2020 Oct 12;21(11):e50085. doi: 10.15252/embr.202050085

Figure 4. β3‐AR agonist activates glucose oxidation in differentiated primary brown adipocytes.

Figure 4

  • A
    Fully differentiated brown adipocytes were treated with β3‐AR agonist CL316,243 (10 μM). Relative mRNA levels of thermogenic markers were measured by qPCR.
  • B, C
    (B) Pyruvate and lactate enrichment, (C) Citrate and malate enrichment were analyzed by GC/MS, after cultured with medium containing 10 mM [U‐13C]glucose and 10 μM CL316,243 for 2 h.
  • D
    Results of metabolic flux analysis (MFA) of primary brown adipocytes treated with or without CL316,243. Arrows are weighted according to absolute flux magnitudes. See Appendix Table S1 for definitions of quantitative flux values, and the units of the flux values are nmol/h/μg protein. OAA, oxaloacetate.
Data information: n = 3 biological repeats, data are represented as the mean ± SD. Statistical analysis was performed using two‐tailed Student's t‐test (A), two‐way ANOVA followed by Tukey's multiple comparisons test (B and C), *P < 0.05. Source data are available online for this figure.