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. 2020 Oct 12;21(11):e50085. doi: 10.15252/embr.202050085

Figure 5. MPC inhibition blocks oxidative metabolism in brown adipocytes and BAT.

Figure 5

  1. Oxygen consumption rate (OCR) of mouse brown adipocytes treated with MPC inhibitor CHC (2 mM) or UK5099 (2 μM), n = 6–7 biological repeats.
  2. Differentiated mouse brown adipocytes were pre‐treated with β3‐AR agonist CL316,243 for 4 h, and then cultured with medium containing 10 mM [U‐13C]glucose for 2 h with CHC or UK5099. Metabolic 13C enrichments in brown adipocytes were shown as m+3 glycolytic intermediates, m+2, and m+3 TCA cycle intermediates, n = 3 biological replicates.
  3. After housed at 6°C for 10 days, mice were IP injected with PBS or CHC (500 mg/kg). 30 min after CHC treatment, mice were IP administered with [U‐13C]glucose (2 g/kg). Metabolic 13C enrichments in BAT of male mice are shown as m+3 glycolytic intermediates, and m+2 and m+3 TCA cycle intermediates, n = 7.
  4. Body temperature of the CHC (left: 400 mg/kg, n = 5) and (right: 500 mg/kg, n = 7) treated mice.
Data information: Data are represented as the mean ± SD. Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparisons test (A and B), two‐tailed Student's t‐test (C), and two‐way ANOVA followed by Sidak's multiple comparisons test (D), *P < 0.05. Source data are available online for this figure.