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. 2020 Sep 7;21(11):e50845. doi: 10.15252/embr.202050845

Figure 4. Transcriptome regulation in response to respiratory inhibition and arginine supplementation.

Figure 4

  1. Changes in transcript abundance 30 and 120 min after addition of antimycin A to exponentially growing cells without (EMM) or with arginine (EMM + R). Transcripts annotated as up‐ or down‐regulated core environmental stress response (CESR) are indicated in red and blue, respectively. All data are normalised to EMM time point 0.
  2. Graphs as in (A) showing expression profiles of transcript encoding enzymes for arginine catabolism (blue) or arginine biosynthesis (red).
  3. Heat map of expression changes for transcripts repressed over 2‐fold at 30 min in cells supplemented with arginine (EMM + R) relative to cells without supplementation (EMM). Values for timepoints 0 and 30 min after antimycin A addition are shown for cells grown in EMM, EMM with arginine and ssp1 deletion mutant grown in EMM. The data are normalised to EMM timepoint 0.
  4. Heat map of expression changes of the same genes as in (B) after 1 h of inhibiting TOR kinase with torin1 or caffeine/rapamycin treatment relative to cell before treatment; cells were grown in rich media—YES (data from (Rodríguez‐López et al, 2019)).
  5. Overlaps between genes repressed with arginine supplementation and genes induced in tor2 thermo‐sensitive mutant (P < E‐27) (Matsuo et al, 2007), or repressed in strains deleted for the TOR inhibitors tsc1 and tsc2 (P < E‐16) (Van Slegtenhorst et al, 2004).
  6. Enriched and underrepresented amino acids in proteins encoded by 40 genes that are down‐regulated in media with arginine. Analysis performed using online tool AnGeLi (Bitton et al, 2015).