Table 6.

Studies of viability of coronaviruses in simulated aerosols.

Coronavirus strain	Viability at different temperature and RH conditions	Type of spraying media	Aerosolization system	Collection system	study	Year
229E (HCV/229E)	At 20 ± 1 °C and 50% RH (half-life of 67.33 ± 8.24 h), at 20 ± 1 °C and 30% RH (half-life of 26.76 ± 6.21 h), at 20 ± 1 °C and 80 ± 5% RH (half-life of 3 h)	Tryptose phosphate broth with 2.5 mg of rhodamine B per mL	Collison nebulizer	Glass impinger	M. K. IJAZ et al. (Ijaz et al., 1985)	1985
MERS-CoV	At 20 °C and 40% RH 7% reduction, at 20 °C and 70% RH 89% reduction	Unknown media	Collison nebulizer	Glass impinger	N van Doremalen1 et al. (van Doremalen et al., 2013)	2013
MERS-CoV	At 25 °C and 79% RH 63.5% survival after 60 min, at 38 °C and 24% RH 4.7% survival after 60 min	Unknown media	Collison nebulizer	Personal bioaerosol samplers with 40 mL of collecting liquid and 4 L/min flow rate	Oleg V. Pyankov et al. (Pyankov et al., 2018)	2017
SARS-CoV	At 21–23 °C and 65% RH half time of 3 h	Unknown media	Collison nebulizer	Viruses were collected on a 47 mm gelatine filter and then dissolved in 10 mL of DMEM containing 10% FBS	Neeltje van Doremalen et al. (van Doremalen et al., 2020)	2020
SARS-CoV-2	At 21–23 °C and 65% RH half time of 3 h	Unknown media	Collison nebulizer	Viruses were collected on a 47 mm gelatine filter and then dissolved in 10 mL of DMEM containing 10% FBS	Neeltje van Doremalen et al. (van Doremalen et al., 2020)	2020