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RD reduces proinflammatory cytokine levels in HFD-fed mice. (A-E) Serum LPS (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-10 (E) levels were determined using ELISA at week 12. N=12 mice per group. Data are presented as mean ± SEM. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Bonferroni's post hoc test. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns, not statistically significant.
