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. 2020 Sep 23;17(17):2735–2743. doi: 10.7150/ijms.51329

Figure 4.

Figure 4

HCP5 functions as a ceRNA by sponging miR-27-3p. (A) The ceRNA network of HCP5 was analyzed by the lnCAR webtool. (B) HCP5 knockdown significantly increased the level of miR-27b-3p in DLBCL cells. (C) Geniposide treatment (500 μM) for 24 h prominently upregulated the expression of miR-27b-3p in DLBCL cells. (D) Luciferase reporter vectors containing wild type (WT) or mutant type (MUT) HCP5 and miR-27-3p mimics or negative control (NC) were cotransfected into OCI-LY7 and OCI-LY3 cells, after which the relative luciferase activity was assessed. (E) The levels of miR-27b-3p in 48 DLBCL samples and 14 reactive lymph node hyperplasia (RLH) specimens were determined by qRT-PCR. (F) An inverse correlation between HCP5 and miR-27b-3p expression was observed in DLBCL tissues. (G) OCI-LY7 and OCI-LY3 cells were transfected with corresponding vectors and subjected to western blotting for MET expression. *P<0.05.