Table 1.

UPLC®-MS procedures and conditions used for each platform

	Platform 1	Platform 2	Platform 3
Column type	UPLC BEH C18, 1.0 × 100 mm, 1.7 μm	UPLC BEH C18, 2.1 × 100 mm, 1.7 μm	UPLC BEH C18, 1.0 × 100 mm, 1.7 μm
Flow rate	0.14 ml/min	0.40 ml/min	0.14 ml/min
Solvent A	H2O + 0.05% Formic Acid	H2O + ACN + 10 mM Ammonium Formate	10 mM Ammonium Bicarbonate (pH= 8.8)
Solvent B	ACN + 0.05% Formic Acid	ACN+ Isopropanol + 10mM Ammonium Formate	ACN
(%B), time	0%, 0 min	40%, 0 min	2%, 0 min
(%B), time	50%, 2 min	100%, 10 min	8%, 6.5 min
(%B), time	100%, 13 min	40%, 15 min	20%, 10 min
(%B), time	0%, 18 min	40%, 17 min	30%, 11 min
(%B), time	-	-	100%, 12 min
(%B), time	-	-	2%, 14 min
Column temperature	40 °C	60 °C	40 °C
Injection volume	2 μl	3 μl	2 μl
Source temperature	120 °C	120 °C	120 °C
Nebulisation N2 flow	600 l/hour	1000 l/hour	600 l/hour
Nebulisation N2 temperature	350 °C	500 °C	350 °C
Cone N2 flow	30 l/hour	30 l/hour	10 l/hour
Capillary voltage	2.8 kV	3.2 kV	3.2 kV
Cone voltage	50 V	30 V	30 V