Figure 2. Embryonic phenotypes of smyhc1 mutants.

• A
  Morphologies of smyhc1 ^+/+, smyhc1 ^−/+, and smyhc1 ^−/− embryos are grossly normal at 2 days postfertilization (dpf), despite complete paralysis of smyhc1^−/− embryos. In contrast, the smyhc1 ^R673H/+ embryos have notochord kinks in larval stages (arrow head), while smyhc1^R673H/R673H embryos have much more severe notochord kinks that compress and distort the body axis (arrow heads). The smyhc1^R673H/− embryos completely phenocopy smyhc1 ^R673H/R673H embryos.
• B
  Quantification of smyhc1 ^R673H embryonic phenotypes at 2 dpf. Representative examples of fish in each of the four phenotypic groups (normal, mild, moderate, and severe) are shown in Fig EV3.