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. 2020 Nov 5;6:34. doi: 10.1038/s41526-020-00123-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Beas-2B cells were treated by simulated microgravity for 48 h first and then exposed to 0.5 Gy X-ray irradiation. Irradiated cells were fixed at 1 h or 24 h post irradiation for γH2AX foci assay. a Immunofluorescent staining of the γH2AX in the cells exposed to simulated microgravity and/or 0.5 Gy X-rays. b The γH2AX foci yields in the cells exposed to simulated microgravity and/or 0.5 Gy X-rays. The experiments were done in triplicate and error bars indicate SEM. Ctrl, control group; IR, irradiation group; SMG, simulated microgravity group; SMG + IR, simulated microgravity combined with irradiation group. Scale bar represents 50 μm. *Compared with control group. #Compared with IR group. &Compared with SMG group. */#/&: p < 0.05.