Figure 1.

Representative example of differentially fractionated samples of Ostrinia nubilalis homogenized thorax tissue to acquire nuclear and mitochondrial components at 6000 and 13,000 rcf, respectively. Janus Green B staining of the mitochondrial fraction indicated abundant mitochondria (a). Gel electrophoresis of serial-diluted DNA extracted from the mitochondrial and nuclear fractions amplified in duplicate with mitochondrial primers (HCO/LCO;⁵⁹) and nuclear primers (Onapn1-F and -R;⁴⁶) (b). Mitochondrial fraction (13,000 rcf) was PCR amplified with mitochondrial primers (i) and nuclear primers (ii). Nuclear fraction (6000 rcf) was PCR amplified with mitochondrial primers (iii) and nuclear primers (iv). Although nuclear DNA was present in both the mitochondrial and the nuclear fractions, mtDNA was only present in the mitochondrial fraction.