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. 2020 Nov 5;10:19123. doi: 10.1038/s41598-020-76088-0

Figure 4.

Figure 4

Alignment of 60 C-terminal residues from representative NADH Dehydrogenase subunit 4 (ND4) protein sequence accessions. Conserved residues (highlighted) showed ≥ 83.33% identity between species, with the exception of the 29 terminal residues from O. nubilalis AAL66246.1 predicted in the reference mitochondrial genome (AF442957.1). Frameshift in ND4 accession AAL66246.1 predicted from the reference AF442957.1 putatively caused by insertion of a cytosine (C) nucleotide at position 1254 of the ND4 CDS (genome position 8013; Table S2). Companion Maximum-Likelihood (ML) constructed based on the mtREV + F + G model of sequence evolution that maximized the Bayesian Information Criterion (BIC) score at 4929.63, and used an empirically-derived discrete gamma distribution (shape parameter; G) of 0.3988 that minimized the log-likelihood score (-2330.81). Total branch length of 0.56 amino acid changes per site. Species abbreviations: Sfru, Spodoptera frugiperda; Tni; Trichoplusia ni; Epos, Epiphyas postvittana; Mvit, Maruca vitrata; Ostrinia species abbreviations as in Fig. 3).