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. 2020 Nov 5;10:19090. doi: 10.1038/s41598-020-76144-9

Table 1.

Other reports of tunicate cellulose purification strategies and respective yields.

Tunicate feedstock Deproteination Bleaching Pretreatment yield (%)* References
Salpa fusiformis Pronase in buffer NaOH reflux N/A 54
Styela clava KOH NaClO/CH3COOH N/A 26,34,35,55
Halocynthia roretzi KOH NaClO/CH3COOH N/A 56
Halocynthia papillosa NaOH NaClO2 N/A 29
Halocynthia roretzi NaOH NaClO2 N/A 57
Halocynthia roretzi Drasche NaOH H2O2 20 5860
Styela clava NaOH NaClO2/CH3COOH 31 This work
Styela clava

Three step hydrothermal process

1 Prehydrolysis (H2SO4)

2 Kraft Cooking (NaOH/Na2S)

3 Bleaching (NaClO)

Step 1 & 2: 180 °C | 2 h

Step 3: 75 °C | 1 h

40 18
Ciona intestinalis 30 18
Ciona intestinalis 12 10,30,61
Ascidia sp. 3 10
Cynthia roretzi 30 62
Styela plicata 24 10
Halocynthia roretzi 21 10

*Approximate values | N/A = not reported.