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. 2020 Nov 5;11:5614. doi: 10.1038/s41467-020-19436-y

Fig. 4. Protrudin overexpression enhances ER presence at growth cones and this interaction is required for successful axon regeneration.

Fig. 4

a Schematic representation of Protrudin endoplasmic reticulum domain mutants. b Representative images of RTN4 immunofluorescence (green) in the distal axon of neurons expressing the indicated m-Cherry Protrudin constructs (magenta). Scale bar is 10 μm. c, d Quantification of RTN4 fluorescence intensity at the axon tip and shaft (p < 0.0001, Kruskal–Wallis with Dunn’s multiple comparisons test). Error bars represent mean ± SEM. e Percentage of regenerating axons in neurons expressing mCherry-Protrudin domain mutants—FFAT (n = 4 independent experiments, 60 neurons) and TM1-3 (n = 5 independent experiments, 45 neurons) compared to phosphomimetic Protrudin as a positive control (n = 2 independent experiments, 21 neurons), and mCherry as a negative control (n = 3 independent experiments, 41 neurons). Error bars represent mean ± SEM.