Figure 2.

RAN protein interacts with α-synuclein. (A-D) hiPSC-derived NPCs were labeled with anti-RAN and anti-α-syn antibodies. RAN protein co-localized with α-syn signal in the nucleus in all cell-lines, the A53T (A) and SNCA-Tri (C) hiPSC-derived MD NPCs and their isogenic corrected controls, A53T corrected (B) and SNCA-Tri corrected (D). Scale bars: 10 μm. (E) Western blot analysis of homogenates prepared from SH-SY5Y cells co-infected with LV-myc-α-syn and LV-3xFlagRAN. Anti-Flag immunoprecipitates were analyzed by western blotting using anti-myc antibody and anti-myc immunoprecipitates were analyzed by western blotting using anti-Flag antibody. No-antibody Co-IP was performed as controls to ruled out non-specific binding (not shown). Co-IP of the endogenous proteins is demonstrated in Figure 5.