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. 2020 Sep 8;29(18):3081–3093. doi: 10.1093/hmg/ddaa199

Figure 4.

Figure 4

Projections of CN V sensory and hindbrain motor neurons into presumptive mx and md targets are precise in WT and LgDel+/− embryos; however, there is an apparent increase in terminal branching and defasciculation in LgDel+/−. (A) Focal injections of two distinct fluorophore-conjugated biocytins into the presumed mx (green) and md (red) portions of E11.5 WT CNgV label single axon fascicles that project linearly and precisely into Ba1A (mx; green) and Ba1B (md; red). In addition, the proximal (hindbrain) projections from these sensory neurons (inset) bifurcate, with one branch directed anteriorly (a, arrow), and the other posteriorly (p, arrow). The Ba1A hindbrain sensory axon branches (green) are more medial, and the Ba1B branches are more lateral. (B1–3) The terminations of individual distal fascicles tend to be coherent and unbranched; however, some examples of terminal branching (B4) are seen in WT. (C) Focal injection of two distinct fluorophore-conjugated biocytins into presumed mx (green) and md (red) portions of E11.5 LgDel+/− CNgV labels axon fascicles that project to their respective targets, Ba1A and Ba1B. There is, however, terminal defasciculation (box 1, arrows, inset). In addition, in both LgDel+/− and WT, we see aberrant fascicles seen in inappropriate territories (box 2, arrows, inset) at fairly low, but equivalent penetrance in both genotypes. (D1–4) The termini of labeled axon fascicles are more branched (arrowheads) and extend in multiple directions than those in WT.