Table 1.
Progeny testing for recovery of genotypes expected from intercross mating
| Line name intercross parents | Number breeding pairs | Total number litters | Total number progeny | Average number per litter | Number male progeny (%) | Number female progeny (%) | Progeny genotype | ||
|---|---|---|---|---|---|---|---|---|---|
| Number wild-type homozygote (%) | Number mutant heterozygote (%) | Number mutant homozygote (%) | |||||||
| Fan1_del10 X Fan1_del10 | 10 | 33 | 181 | 5.5 | 85 (47.0) | 96 (53.0) | 44 (24.3) | 98 (54.1) | 39 (21.5) |
| Fan1_ins1 X Fan1_ins1 | 4 | 9 | 49 | 5.4 | 26 (53.1) | 23 (46.9) | 10 (20.4) | 30 (61.2) | 9 (18.4) |
| Mtmr10_del5 X Mtmr10_del5 | 5 | 11 | 66 | 6.0 | 38 (57.6) | 28 (42.4) | 16 (24.2) | 35 (53.0) | 15 (22.7) |
| Rrm2b_del10 X Rrm2b_del10 | 6 | 21 | 111 | 5.3 | 63 (56.8) | 48 (43.2) | 23 (20.7) | 59 (53.2) | 29 (26.1) |
| Rrm2b_del24 X Rrm2b_del24 | 2 | 9 | 46 | 5.1 | 27 (58.7) | 19 (41.3) | 9 (19.6) | 24 (52.2) | 13 (28.3) |
| Ubr5_ins2 X Ubr5_ins2 | 2 | 2 | 9 | 4.5 | 5 (55.6) | 4 (44.4) | 0 (0) | 9 (100) | 0 (0) |
| Ubr5_del7 X Ubr5_del7 | 2 | 4 | 11 | 2.75 | 4 (36.4) | 7 (63.6) | 2 (18.2) | 9 (81.8) | 0 (0) |
Summary of breeding data revealed that mutant alleles at Fan1, Mtmr10 and Rrm2b were observed in approximately Mendelian ratios (25% homozygous wild-type, 50% heterozygous mutant, 25% homozygous mutant) in progeny of intercross mating genotyped at weaning, consistent with reported normal development for mice with other mutations inactivating Fan1 and Rrm2b. Mtmr10 null mice have not previously been reported but null mutations in other family members is also not lethal. Progeny homozygous for Ubr5 mutant alleles were not recovered, as this gene (previously Edd) is essential early in development. Mutant alleles were passed to males and females about equally, as expected.