Table 1.
Assembly statistics of S. intermedia clones.
| Sequencing technique | PacBio (clone 7747) | ON/Illumina (clone 8410) |
|---|---|---|
| Input data | ||
| Read coveragea | 37.6× | 191× |
| Chromosome number | 18 | |
| Genome physical sizeb | ~ 160 Mbp | |
| Assembly statistics and gene prediction | ||
| Assembly length (Mbp) | 147.6 | 136.6 |
| Number of pseudomolecules | 18 | |
| Number of contigs | 584 | 86 |
| Number of scaffolds | 420 | 70 |
| Number of assigned scaffolds | 18 pseudomolecules (featuring 63 contigs) ~ 131.4 Mbp total length | 18 pseudomolecules (featuring 34 contigs) ~ 134 Mbp total length |
| Largest scaffold length (Mbp) | 12.5 (Si09) | 13.4 (Si09) |
| N50 scaffold length (Mbp) | 8.3 | 9.25 |
| G + C content (%) | 41.6 | 42.0 |
| Number of predicted gene models | 22,245 | 21,594 |
| Completeness of gene prediction (BUSCO)c | ||
| Complete genes (C) | 1097 (79.8%) | 1280 (93.1%) |
| Complete and single-copy (S) | 1085 (78.9%) | 1266 (92.1%) |
| Complete and duplicated (D) | 12 (0.9%) | 14 (1.0%) |
| Fragmented genes (F) | 131 (9.5%) | 41 (3.0%) |
| Missing genes (M) | 147 (10.7%) | 54 (3.9%) |
| Total number of BUSCO genes used | 1375 | |
aBased on genome size measurements by FCM.
bMeasured by FCM.
cReference database odb10.