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. 2020 Nov 5;10:19128. doi: 10.1038/s41598-020-76129-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(A) Structure of ethinyl estradiol (EE). Image obtained from Wikipedia (https://commons.wikimedia.org/wiki/File:Ethinylestradiol.svg). (B) Serum alkaline phosphatase and total bilirubin levels of animals that are exposed to EE. Graphs are generated by Graphpad Prism8 software (GraphPad Software Inc., La Jolla, CA, www.graphpad.com). (C) Total body weight, liver weight and serum triglyceride levels of animals that are exposed to EE. Graphs are generated by Graphpad Prism8 software (GraphPad Software Inc., La Jolla, CA, www.graphpad.com). (D) Hierarchical clustering of hepatic genes regulated by low-, medium- and high-dose EE exposure at selected time points. Cluster3 software (https://bonsai.hgc.jp/~mdehoon/software/cluster/) was used for clustering the differentially expressed genes. Data was visualized using Treeview Java (https://jtreeview.sourceforge.net/).