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. 2020 Nov 5;11:5598. doi: 10.1038/s41467-020-19251-5

Fig. 5. Condensation activity of BioZ.

Fig. 5

a Scheme for testing elongation of glutaryl-CoA with malonyl-ACP. The unstable product of decarboxylative condensation was converted to pimeloyl-ACP by successive reduction, dehydration, and reduction steps catalyzed by the E. coli fatty-acid synthetic (Fab) enzymes as shown. BioZ is in red font. b Conformationally sensitive urea electrophoretic separation of ACP species. The At, Ba, and Rm BioZ proteins catalyze formation of a product having the mobility of pimeloyl-ACP whereas three different FabH proteins and the Mj and Mh BioZs failed to form the product. This gel and gels in which alternate primers and elongation substrates were tested are given together in Supplementary Figure 3. Each experiment was repeated more than three times and the gel given is representative. Strain abbreviations are given as in Fig. 4.