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. 2020 Nov 5;11:5598. doi: 10.1038/s41467-020-19251-5

Fig. 7. Mass spectral analysis of pimeloyl-ACP synthesis and MS/MS detection of a trapped  active site glutarate adduct.

Fig. 7

Mass spectral analyses of intact ACP species a–d and of the modified active site peptide of the BioZ C115S mutant protein e. a Reaction containing malonyl-ACP and glutaryl-CoA as substrates and lacking AtBioZ b. Reaction containing malonyl-ACP and glutaryl-CoA as substrates supplemented with AtBioZ. c and d tested glutaryl-ACP and malonyl-CoA as substrates with or without SfBioZ addition. e MS/MS spectra of the active site peptide of the AtBioZ C115S purified from an E. coli expression strain. Analysis by the MASCOT program (inset at top right) indicated the presence of glutaryl modifications of serine residues 115 and 116. Traces of pimeloyl modification were also present whereas no malonyl modification was seen.