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. 2020 Nov 5;11:5587. doi: 10.1038/s41467-020-19383-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Protein expression profiles for the top 100 proteins across cell types using specific, well-characterized proteins as reference for each subset. a Protein expression profile of DCs using (b) CD1c as a reference (red line in (a)). Protein expression of selected proteins enriched in DCs (c) NMES1 and (d) CD1b across cell types. e Protein expression profile of MΦ using (f) CD163 as a reference profile (red line in (e)). Protein expression of selected proteins enriched in MΦ (g) MS4A6A and (h) FOLR2 across cell types. i Protein expression profile of MCs using (j) carboxypeptidase A (CPA3) as a reference profile (red line in (i)) and expression of selected proteins enriched in MCs (k) LAT2 and (l) SYTL3) across cell types. m Protein expression profile of ETs and DTs using (n) T-cell receptor chain CD3δ as a reference profile (red line in (m)). Protein expression of selected proteins enriched in ETs and DTs (o) PTPRCAP and (p) SEPTIN1 across cell types. q Protein expression profile of ECs using (r) adhesion molecule cadherin 5 (CDH5) as a reference profile (red line in (q)) and bar plots illustrating the expression of selected proteins enriched in ECs (s) DYSF and (t) KANK3 across cell types. u Protein expression profile of melanocytes (Mel) using (v) adhesion molecule melanogenesis pathway member Melan-A (MELAN-A) as a reference profile (red line in (u)) and bar plots illustrating the expression of selected proteins enriched in melanocytes (w) MTURN and (x) CRYM) across cell types. All barplots are presented as mean±SEM, (log2). DC; CD1A⁺dendritic cells (N = 3), MΦ; macrophages (N = 4), MC; mast cells (N = 6), DT; dermal T cells (N = 6), ET; epidermal T cells (N = 6), EC; endothelial cells (N = 4), Mel; melanocytes (N = 5), Fib; fibroblasts (N = 5), KC; keratinocytes (N = 5). N represents number of biologically independent samples. Source data are provided as a Source Data file.