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. 2020 Nov 5;10:19091. doi: 10.1038/s41598-020-76251-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of vitamin B on NLRP3 inflammasome activation. (A) Schematic diagram of the experimental process of inflammasome activation. Macrophages, such as BMDMs or PMA-treated THP-1 cells, were primed with LPS for 3 h, and the inflammasome activation was then triggered in the presence of a vitamin. The indicators of the inflammasome activation were analyzed by ELISA or Western blotting. (B) LPS-primed BMDMs activated the NLRP3 inflammasome with NG with/without a vitamin as indicated. The secretion of IL-1β was measured by ELISA. (C) LPS-primed BMDMs were treated with a vitamin, as indicated without the inflammasome triggers. The ATP treated group was used as a positive control. (D) LPS-primed BMDMs were treated a vitamin, as indicated for 24 h, and the cytotoxicity was analyzed. The survival rates of the non-treated cells were set to 100%, and the cells treated with triton X-100 (Triton-X, 0.01%) were regarded as 0% viability. The bar graph presents the mean ± SD with at least two independent experiments.