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. 2020 Nov 5;10:19183. doi: 10.1038/s41598-020-76302-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy images of bacterial aggregates and EPS from Yungay subsurface clay samples. (a, b), Maximum image projection 3D images (upper and right bars: X and Y view of stacked sections respectively, 10 sections; z = 0.35 μm) of SYBR green stained bacterial cells (green-yellow signal) and autofluorescence red signal (arrow), potentially proceeding from exopolysaccharides. The sGFP and Rhodamine filters were used for both images (Multichannel Image Acquisition system). (c) Single section image of SYBR green stained bacterial cells (green signal) and autofluorescence orange (arrow) signal potentially proceeded from EPS; sGFP filter. (d) Single section image of SYBR green stained bacterial cells (blue signal) and autofluorescence green (arrow) signal potentially proceeded from EPS; DAPI filter. (e, f), Maximum Image Projection 3D images (upper and right bars: X and Y view of stacked sections respectively, 10 and 15 sections respectively; z = 0.35 μm) of CTC stained and metabolically active bacterial cells (yellow-orange CTF signal).