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. 2020 Oct 28;21(12):e51252. doi: 10.15252/embr.202051252

Figure 1. Alveolar macrophages are highly responsive to IFN‐λ and good producers of IFNs.

Figure 1

  • A–C
    (A) Total RNA was harvested and subjected to qPCR for quantification of the IFN response measured by IFNA2, IFNB1 and IFNL1. (B) ISG response measured by ISG15 and RSAD2. (C) Quantification of the presence of SeV or IAV. (A–C) Each colour corresponds to a donor; 9 different donors were analysed. The dashed line represents the detection limit. An ordinary one‐way ANOVA test was used for the statistical analysis: ns, not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

Alveolar macrophages from different donors were purified from BAL fluid by allowing them to attach to the culture dish for 2 h. Subsequently, they were pre‐treated overnight with either type I or type III IFNs (1 ng/ml IFN‐λ1 and 10 U/ml IFN‐α2, respectively) before infection with Sendai virus (SeV) and influenza A virus (IAV, strain PR8) for 6 h as indicated. The column labelled c shows control samples collected directly after purification.

Source data are available online for this figure.
 Source data are available online for this figure.