Figure 2. Alveolar macrophages do not produce an IFN response upon SARS‐CoV‐2 challenge.

• A–D
  Alveolar macrophages from different donors were purified from BAL fluid and pre‐treated with either Poly(I:C) or IFN‐λ1 (+; 1 ng/ml, or ++; 10 ng/ml) before infection with SARS‐CoV‐2. (A) Total RNA was harvested and subjected to qPCR for quantification of the IFN response measured by IFNA2, IFNB1 and IFNL1 (Donors marked in orange and blue were not quantifiable in all test conditions). (B) ISG response measured by ISG15, RSAD2 and IFIT1. (C) Quantification of the presence of SARS‐CoV‐2. (D) An overview of the timeline of the experiment. (A–C) Each colour corresponds to a donor; 6 different donors were analysed. The dashed line represents the detection limit. An ordinary one‐way ANOVA test was used for statistical analysis: ns, not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

Source data are available online for this figure.
 Source data are available online for this figure.